Comprehensive Coverage of Exosome Purification and Exosomal RNA Isolation from Different Types of Liquid Biopsies
Speaker: Bastien Paré, MSc with Dr Moemen Abdalla, MSc, PhD
Summary: Evidence has been accumulating recently that Extracellular vesicles (EVs), especially exosomes, act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes and other extracellular vesicles (EVs) which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNA may serve as biomarkers for various diseases including cancer. Recent work has demonstrated that currently available exosome purification methods may yield a different set of exosome based on the approach of purifying these vesicles. Since the RNA content of these exosomes is mainly small RNA (miRNA) and found in very low amounts, the RNA isolation method is very critical. Some current exosomal RNA isolation methods have either bias towards binding certain sizes and/or specific RNA sequences depending on their GC contents. In this webinar we will be discussing the advantages and disadvantages of the different exosomes purification methods as well as the different RNA isolation methods from different sample types. We will be also discussing the different exosomal RNA quantification methods and some of the downstream applications associated with exosomal RNA analysis such as Next Generation Sequencing.
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