The cells continuously secrete a large number of different types of microvesicles including macro-and micro-molecules into the extracellular fluids. One of them are exosomes, which are nano-sized vesicles capable of transferring the DNAs, microRNAs, non-coding RNAs and lipids with or ...Read More »
Urinary exosomes and microvesicles (EMV) are promising biomarkers for renal diseases. Although the density of EMV is very low in urine, large quantity of urine can be easily obtained. In order to analyze urinary EMV mRNA, a unique filter device ...Read More »
Urine is a readily available source of relatively large quantities of extracellular vesicles (EVs). However, the isolation of urinary EVs (uEVs) is complicated by the presence of Tamm-Horsfall protein (THP), which polymerizes and co-precipitates as a contaminant. This may make ...Read More »
from abcam.com Various secreted extracellular vesicles are present in urine, amniotic fluid, bronchoalveolar lavage fluid, breast milk, saliva and bloodRead More »
Isolation and Quantification of MicroRNAs from Urinary Exosomes/Microvesicles for Biomarker Discovery
Recent studies indicate that microRNA (miRNA) is contained within exosome. Here researchers from Southeast University, China sought to optimize the methodologies for the isolation and quantification of urinary exosomal microRNA as a prelude to biomarker discovery studies. Exosomes were isolated ...Read More »
Urinary exosome mRNA of CD2AP may be a non-invasive tool for detecting both renal function and fibrosis of kidney disease.
Podocyte injury plays an important role in the pathogenesis of kidney disease. Urinary exosomes are microvesicles released by tubular epithelial cells and podocytes containing information of their originated cells. This study investigated for the first time whether podocyte related mRNA ...Read More »