Tag Archives: ultracentrifugation

The purity of exosomes required for diagnostic profiling of small RNA suitable for biomarker discovery

exosomes

Small non-coding RNAs (ncRNA), including microRNAs (miRNA), enclosed in exosomes are being utilised for biomarker discovery in disease. Two common exosome isolation methods involve differential ultracentrifugation or differential ultracentrifugation coupled with Optiprep gradient fractionation. Generally, the incorporation of an Optiprep ...

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MicroRNA Profiling of Exosomes

exosomes

Exosomes are nano-sized membrane-bound vesicles released by a range of different cell types. Exosomes have been shown to specifically package certain membrane and cytosolic proteins and nucleic acids. Furthermore, it has been shown that their contents can be transferred to ...

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Isolation of Exosomes from Blood Plasma – Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods

exosome rna

Exosomes are emerging targets for biomedical research. However, suitable methods for the isolation of blood plasma-derived exosomes without impurities have not yet been described. Therefore, researchers from Semmelweis University, Hungary investigated the efficiency and purity of exosomes isolated with potentially ...

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