Amplify Exosome RNAs for Profiling
RNAs present in patient body fluids are a rich and untapped source of disease-related biomarkers. The RNAs are stable in serum because they are encapsulated in circulating exosomes. Exosomes are 60–120 nm membrane vesicles secreted by most cell types in vivo and in vitro. Exosomes are found in blood, urine, amniotic fluid, malignant ascite fluids and contain distinct subsets of microRNAs depending upon the tumor or tissue from which they are secreted. The SeraMir kit includes everything needed to accurately and sensitively measure RNAs from serum samples. Exosomes are efficiently isolated using SBI’s ExoQuick solution, and the exoRNAs are purified using a phenol-free lysis buffer and rapid spin columns. The SeraMir kit enables the 3’ tailing and simultaneous tagging of both 5’ and 3’ ends during cDNA synthesis—ready for qPCR. Primers for PCR amplification are included to make double-stranded cDNA that is ready to generate sense-strand exoRNAs using for T7 IVT. Use the amplified exoRNAs for microarrays and NextGen sequencing applications.
Go from serum sample to amplified exoRNAs in one day