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  1. Manoj R.Medapati, AnulaSingh, Ranjit R.Korupally, DanaHenderson, ThomasKlonisch, Sasidhar V.Manda, PrashenChelikani. Characterization of GPCRs in extracellular vesicle (EV). Methods in Cell biology, Volume 142, 2017, pages 119-132

    Extracellular vesicle (EV) are tiny membranous vesicles usually < 500 nm in size that recently emerged as a new paradigm in human intercellular signaling. EVs have shown a promising role in development of diagnostic markers in many pathophysiological disorders. The presence of chemosensory and therapeutically relevant G protein-coupled receptors (GPCRs) on EV membranes is poorly characterized. Here, we compare different methods including ultracentrifugation and polymer-charge-based separation to isolate EVs from cell culture media and human saliva. The presence of bitter taste GPCRs (T2R4 and T2R38) and a class A GPCR angiotensin II type 1 receptor on these EVs was characterized by qPCR, ELISA, and immunotransmission electron microscopy.

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