Size-dependent cellular uptake of exosomes

exosomes

The ability of exosomes to elicit specific cellular responses suggests that they may be increasingly used as therapeutics. Their vesicular nature makes them suitable as potential nanocarriers for drugs or nucleic acids delivery. Here researchers from the University of Lincoln address the question whether the method of preparation of enriched exosomal fractions can affect their uptake by cells and their ability to trigger a response. They compared ultracentrifugation and polymer-based precipitation methods on supernatants of glioma-associated stem cells isolated from a high-grade glioma patient. They determined particle size distributions after purification and their correlation with uptake, proliferation and migration in glioblastoma cell cultures. These findings indicate that polymer-based precipitation leads to smaller particle size distributions, faster uptake by target cells and increased cellular motility. The different effect that isolation method-dependent populations of particles have on cell motility suggests their size distribution could also profoundly affect exosome therapeutic potential.

Caponnetto F, Manini I, Skrap M, Palmai-Pallag T, Di Loreto C, Beltrami AP, Cesselli D, Ferrari E. (2016) Size-dependent cellular uptake of exosomes. Nanomedicine [Epub ahead of print]. [abstract]

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