New Products for Exosome Isolation to mRNA Purification

MOUNTAIN VIEW, Calif., May 1, 2015 /SATPR.COM/ — Hitachi Chemical Diagnostics, Inc. is pleased to announce the United States launch of the ExoComplete™ 96-Well Plate Kit and ExoComplete™ Tube Kit, an integrated, fast and easy system for exosome collection to mRNA purification.  The products were introduced during the International Society for Extracellular Vesicles (“ISEV”) Annual Meeting, April 23 – 26, 2015, in Washington, D.C.  Both products are intended for research use only of molecular biology applications and are neither intended for the diagnosis, prevention, or treatment of a disease, nor has either been validated for such use either alone or in combination with other products.

The ExoComplete™ system isolates exosomal mRNA from biological samples such as plasma in a 96-well filter plate format1, or large-volume samples such as urine in a single collection tube format2.  Exosomes and microvesicles (EMVs) in body fluids are captured with Hitachi Chemical’s Exosome Isolation Plate or Exosome Collection Tube with a proprietary filter material.  The highly porous material allows fast filtration of biological samples without clogging, and reproducible isolation of EMVs without the use of conventional ultracentrifugation.  After lysing the captured EMVs, mRNA can be isolated through hybridization with single strand oligo (dT) immobilized in the wells of the 96-well mRNA Capture Plate.  A wide variety of downstream applications become available following isolation of mRNA, including mRNA quantification, single and double-stranded cDNA synthesis, RT-PCR, real time quantitative RT-PCR (qPCR), and next-generation RNA sequencing.

Hitachi Chemical Diagnostics, Inc., a division of Hitachi Chemical Company America, Ltd., Cupertino, California is headquartered in the heart of Silicon Valley in Mountain View, California, and is a global leader of multiplex in vitro allergy diagnostics.  The company works with industry leaders, laboratories, and distributors in more than 40 countries. The company holds many in vitro allergy testing technology patents and continues to pioneer advancements in the field.  Visit www.hcdiagnostics.com to learn more. 

  1. Aoki, J. et al. Posttransplantation Bone Marrow Assessment by Quantifying Hematopoietic Cell-Derived mRNAs in Plasma Exosomes/Microvesicles. Clin. Chem. clinchem.2013.213850 (2014)
  2. Murakami, T. et al. Development of Glomerulus-, Tubule-, and Collecting Duct-Specific mRNA Assay in Human Urinary Exosomes and Microvesicles. PLoS ONE 9, e109074 (2014)

Source – PRNewswire

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