Label-free quantitative proteomic analysis of human plasma-derived microvesicles

To find potential biomarkers of Abdominal Aortic Aneurysms (AAA) researchers from the IPBS, France performed a differential proteomic study based on human plasma-derived microvesicles. Exosomes and microparticles isolated from plasma of AAA patients and control subjects (n = 10 each group) were analyzed by a label-free quantitative mass spectrometry (MS)-based strategy. Homemade and publicly available software packages have been used for MS data analysis.

The application of two kinds of bioinformatic tools allowed us to find differential protein profiles from AAA patients. Some of these proteins found by the two analysis methods belong to main pathological mechanisms of AAA such as oxidative stress, immune-inflammation and thrombosis. Data analysis from label-free MS-based experiments requires the use of sophisticated bioinformatic approaches to perform quantitative studies from complex protein mixtures. The application of two of these bioinformatic tools provided us a preliminary list of differential proteins found in plasma-derived microvesicles not previously associated to AAA, which could help us to understand the pathological mechanisms related to this disease.

Martinez-Pinna R1, de Peredo AG, Monsarrat B, Burlet-Schiltz O, Martin-Ventura JL. (2014) Label-free quantitative proteomic analysis of human plasma-derived microvesicles to find protein signatures of Abdominal Aortic Aneurysms. Proteomics Clin Appl [Epub ahead of print]. [abstract]

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