Type: Full Time
Location: Boston, Massachusetts
PRINCIPAL DUTIES AND RESPONSIBILITIES:
- Work closely with the PI/postdoctoral fellows on a biomedical research project funded by the Marr grant to study the roles of exosomes, small cell-derived extracellular vesicles, in neurodegenerative disease.
- Be responsible for culturing cells which will be the primary source of exosomal material.
- Isolate exosomes from biological materials using ultracentrifugation, immunoisolation and chemical precipitation to monitor their size, morphology and general protein profile.
- Screen purified exosomes for proteins directly relevant to neurodegenerative disease using SDS-PAGE, ELISA and electron microscopy.
- Record and analyze results using the appropriate software packages.
- Prepare buffers, reagents place orders to keep the lab stocked of necessary consumables.
SKILLS/ ABILITIES/ COMPETENCIES REQUIRED:
- Bachelors degree in biochemistry, neuroscience, cell biology or a related biomedical subject.
- The successful candidate should also have at least 1 year experience in a laboratory setting.
- The successful candidate will be familiar with basic biochemical and cell biological procedures, including buffer preparation, protein assays, electrophoresis, ELISA and cell culture.
WORKING CONDITIONS: Biomedical laboratory setting.
FISCAL RESPONSIBILITIES: Placing orders for lab consumables.
Brigham and Womens Hospital is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, sex, color, religion, national origin, sexual orientation, protected veteran status, or on the basis of disability.