Biological Dynamics announced the publication of data in ACS Nano demonstrating that the company’s proprietary lab-on-a-chip ExoVerita™ system can simplify and streamline the process for isolation and recovery of exosomes. The company is using this technology to develop a portfolio of minimally-invasive diagnostic tests to provide faster answers to critical clinical questions in high-burden diseases, such as cancer, traumatic brain injury, and infectious diseases.
Exosomes are cell-derived, extracellular vesicles that enable communication between cells. They are secreted from most cell types and released in bodily fluids such as urine, blood plasma, and saliva. Due to their stability and ability to transport information about their origin and the state of their parental cells, exosomes are believed to have great potential to power the next generation of liquid biopsies and cancer biomarkers.
“Current exosome isolation methods are generally expensive, complex and cumbersome, which could limit large-scale diagnostic applications,” said Michael Heller, Ph.D., principal investigator on the paper and scientific advisory board member of Biological Dynamics. “This study describes a relatively simple, rapid, and non-destructive method for the isolation of exosomes, that preserves their valuable biomarker information for direct analysis. The technology is setting the stage for rapid, seamless sample-to-answer liquid biopsy, cancer therapy monitoring, and ultimately early disease detection.”
In the study published last week, the researchers evaluated human breast cancer patient plasma and plasma samples spiked with glioblastoma exosomes using the ACE (ExoVerita) system. Despite the use of a relatively small sample volume (<50 microliters), the system was able to successfully capture exosomes in 30 minutes, while preserving the integrity of RNA, transmembrane, and internal proteins. The research team also eluted the exosomes off the chip and confirmed that the extraction process provides viable mRNA for qPCR/RT-PCR analysis.
“This data supports our confidence in the potential for our platform to develop important molecular diagnostics to improve the detection and treatment of cancer,” said Raj Krishnan, Ph.D., CEO of Biological Dynamics. “This study, along with previous publications, demonstrates our technology can isolate and analyze exosomal DNA, RNA, and proteins at the same time. By providing one platform to access the three pillars of diagnostics, we are positioned to develop a new generation of multimodal diagnostics to transform the field.”
The paper entitled “Rapid Isolation and Detection of Exosomes and Associated Biomarkers from Plasma” was published online on July 3 and can be found here: http://pubs.acs.org/doi/pdf/10.1021/acsnano.7b00549.
Source – PR Newswire