A New Assay to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids

The pregnancy-associated disease preeclampsia is related to the release of syncytiotrophoblast extracellular vesicles (STBEV) by the placenta. To improve functional research on STBEV, reliable and specific methods are needed to quantify them. However, only a few quantification methods are available and accepted, though imperfect. For this purpose, we aimed to provide an enzyme-linked sorbent assay (ELSA) to quantify STBEV in fluid samples based on their microvesicle characteristics and placental origin.

The described ELSA is a rapid and simple method to quantify STBEV in diverse liquid samples, such as blood or perfusion suspension. The reliability of the ELSA was proven by comparison with nanoparticle tracking analysis.

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Göhner C, Weber M, Tannetta DS, Groten T, Plösch T, Faas MM, Scherjon SA, Schleußner E, Markert UR, Fitzgerald JS. (2015) A New Enzyme-linked Sorbent Assay (ELSA) to Quantify Syncytiotrophoblast Extracellular Vesicles in Biological Fluids. Am J Reprod Immunol [Epub ahead of print]. [abstract]

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